Regulation of meiotic cell cycle:
Cell division is a highly regulated process, driven by the action of cyclin-dependent kinases (Cdks) and their regulatory subunits, cyclins. Inappropriate activation of Cdk/cyclin complexes can drive unregulated cell division resulting in cell death or tumor formation. Cdk/cyclin complexes also play essential roles in a variety of cellular processes such as development and differentiation. Our lab is focused on the action of Cdk/cyclin complexes and all pathways that control or are controlled by Cdk/cyclin complexes during female meiosis.
Homologous chromosome segregation and aneuploidy:
Reduction of chromosome number during meiosis is essential for producing haploid gametes from diploid parental cells. This reduction is achieved by two successive rounds of chromosome segregation, meiosis I (MI) and meiosis II (MII), after a single round of DNA replication. Although MII resembles mitosis in that sister chromatids separate and segregate to different daughter cells, the pattern of chromosome segregation during MI is unique. During MI, homologous chromosomes pair and then segregate from each other. Defects in this process result in aneuploidy, leading to miscarriages, infertility and genetic disorders such as Down’s syndrome. Therefore, our lab studies the molecular mechanisms that control homologous chromosome segregation during meiosis.
Control of oocyte quality:
Mammalian oocytes are arrested at the prophase of the first meiosis. After LH surge, the oocytes resume meiosis. During these processes, the oocytes is subjected to various sources of damage-inducing factors, which may lead to a progressive deterioration of oocyte quality. Thus, the control of oocyte quality is critical to reproductive success and survival of a species; however, the precise mechanisms underlying this process remain elusive. Our lab is interesting in identifying the molecular mechanisms that control oocyte quality and eventually leading to identification of diagnostic markers that are predictive of oocyte quality in a clinical setting.
Chromatin remodeling during fertilization:
Fertilization leads to the transformation of two haploid gametes into a totipotent zygote. During this process, highly condensed chromatins are largely reorganized, preparing to initiate the transcription of embryonic genes which are essential for obtaining the pluripotency. Our lab is exploring the mechanisms of the chromatin remodeling upon fertilization and investigating the role of maternal factors in this process.
Experience
2012 ~ Present: Assistant and Associate Professor, Department of Integrative Biotechnology, Sungkyunkwan University, Korea
2007 ~ 2012: Postdoctoral fellow, Center for Reproductive Science, University of California San Francisco (UCSF), USA
Journal Articles
(2024)
Distinct characteristics of the DNA damage response in mammalian oocytes.
EXPERIMENTAL AND MOLECULAR MEDICINE.
56,
2
(2023)
Endosulfine alpha maintains spindle pole integrity by recruiting Aurora A during mitosis.
BMC CANCER.
23,
00
(2023)
Oocytes can repair DNA damage during meiosis via a microtubule-dependent recruitment of CIP2A-MDC1-TOPBP1 complex from spindle pole to chromosomes.
NUCLEIC ACIDS RESEARCH.
51,
10
(2022)
Selective utilization of non-homologous end-joining and homologous recombination for DNA repair during meiotic maturation in mouse oocytes.
CELL PROLIFERATION.
56,
4
(2022)
Azoxystrobin exposure impairs meiotic maturation by disturbing spindle formation in mouse oocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY.
10,
-
(2022)
ROS-independent cytotoxicity of 9,10-phenanthrenequinone inhibits cell cycle progression and spindle assembly during meiotic maturation in mouse oocytes.
JOURNAL OF HAZARDOUS MATERIALS.
436,
-
(2022)
The Capacity to Repair Sperm DNA Damage in Zygotes is Enhanced by Inhibiting WIP1 Activity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY.
10,
-
(2022)
MDC1 is essential for G2/M transition and spindle assembly in mouse oocytes.
CELLULAR AND MOLECULAR LIFE SCIENCES.
79,
4
(2021)
Increased WIP1 Expression With Aging Suppresses the Capacity of Oocytes to Respond to and Repair DNA Damage.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY.
9,
(2021)
TCTP overexpression reverses age-associated telomere attrition by upregulating telomerase activity in mouse oocytes.
JOURNAL OF CELLULAR PHYSIOLOGY.
237,
1
(2021)
Discovery and Characterization of a Novel MASTL Inhibitor MKI-2 Targeting MASTL-PP2A in Breast Cancer Cells and Oocytes.
PHARMACEUTICALS.
14,
7
(2020)
RASSF1A Regulates Spindle Organization by Modulating Tubulin Acetylation via SIRT2 and HDAC6 in Mouse Oocytes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY.
8,
601972
(2020)
MKI-1, a Novel Small-Molecule Inhibitor of MASTL, Exerts Antitumor and Radiosensitizer Activities Through PP2A Activation in Breast Cancer.
FRONTIERS IN ONCOLOGY.
10,
(2020)
Mis12 controls cyclin B1 stabilization via Cdc14B-mediated APC/C-Cdh1 regulation during meiotic G2/M transition in mouse oocytes.
DEVELOPMENT.
147,
8
(2019)
Prevention of quality decline and delivery of siRNA using exogenous TCTP translocation across the zona pellucida in mouse oocytes.
SCIENTIFIC REPORTS.
9,
18845
(2019)
Melatonin protects mouse oocytes from DNA damage by enhancing nonhomologous end-joining repair.
JOURNAL OF PINEAL RESEARCH.
67,
4
(2019)
Identification of a novel embryo-prevalent gene, Gm11545, involved in preimplantation embryogenesis in mice.
FASEB JOURNAL.
33,
10
(2019)
Zw10 is a spindle assembly checkpoint protein that regulates meiotic maturation in mouse oocytes.
HISTOCHEMISTRY AND CELL BIOLOGY.
152,
3
(2018)
MASTL inhibition promotes mitotic catastrophe through PP2A activation to inhibit cancer growth and radioresistance in breast cancer cells.
BMC CANCER.
18,
(2018)
Centrosome Clustering Is a Tumor-selective Target for the Improvement of Radiotherapy in Breast Cancer Cells.
ANTICANCER RESEARCH.
38,
6
Patent/Intellectual Property
Translationally controlled tumor protein (TCTP)의 항노화 효과를 통한 난자의 질 개선 및 유지 효과.
10-2016-0100569.
20180306.
KOREA, REPUBLIC OF
애엽 추출물을 포함하는 난모세포(oocytes) 노화 방지용 조성물.
10-2016-0076695.
20171108.
KOREA, REPUBLIC OF
Conference Paper
(2021)
Novel regulatory mechanisms of DNA damage response and meiotic resumption in mouse oocytes.
Gordon Research Conference.
UNITED STATES
(2020)
RASSF1A regulates spindle organization by modulating tubulin acetylation via SIRT2 and HDAC6 in mouse oocytes.
Development and Reproduction.
KOREA, REPUBLIC OF
(2020)
Aging-associated increase in WIP1 phosphatase decreases a capacity of oocytes to repair DNA damage.
KSMCB.
KOREA, REPUBLIC OF
(2019)
Mammalian eggs have a capacity to repair double-strand break-induced DNA damage before fertilization.
2019 ICKSMCB.
KOREA, REPUBLIC OF
(2019)
Mis12 is required for meiotic resumption but dispensable for progression through meiosis I and meiosis II.
2019 ICKSMCB.
KOREA, REPUBLIC OF
(2019)
Prevention of Quality Decline and Delivery of siRNA using Exogenous Translationally-controlled tumor protein that can internalize into the Mouse oocytes.
2019 ICKSMCB.
KOREA, REPUBLIC OF
(2019)
Melatonin protects mouse oocytes from DNA damage by enhancing non-homologous end-joining repair.
Development and Reproduction.
KOREA, REPUBLIC OF
(2019)
Novel regulatory mechanisms of meiotic resumption in mouse oocytes.
Development and Reproduction.
KOREA, REPUBLIC OF
(2018)
Protective Effect of Melatonin against DNA Damage in Mouse Oocytes.
The 30th International Conference of the Korean Society for Molecular and Cellular Biology.
KOREA, REPUBLIC OF
(2018)
Translationally Controlled Tumor Protein Has an Anti-aging Effect during Postovulatory Aging after Penetrating the Zona-pellucida of Oocyte.
The 30th International Conference of the Korean Society for Molecular and Cellular Biology.
KOREA, REPUBLIC OF
(2017)
Inhibition of Wip1 phosphatase sensitizes mouse oocytes to DNA damage.
2017 International Conference Korean Society for Molecular and Cellular Biology (ICKSMCB).
KOREA, REPUBLIC OF
(2016)
TCTP is a critical factor to prevent oocyte aging by regulating microtubule dynamics in mouse.
대한생식의학회 추계 학술대회.
KOREA, REPUBLIC OF
(2016)
Mis12 is essential for asymmetric division by regulating time of polar body extrusion.
International Conference of the Korean Society for Molecular and Cellular Biolgoy (ICKSMCB).
KOREA, REPUBLIC OF
(2016)
Zw10 is required for accurate chromosome segregation and SAC activity in meiosis.
International Conference of the Korean Society for Molecular and Cellular Biology (ICKSMCB).
KOREA, REPUBLIC OF
(2015)
Beclin-1 participates in cytokinetic abscission during oocyte meiosis.
International Conference of the Korean Society for Molecular and Cellular Biology.
KOREA, REPUBLIC OF
(2015)
CIP2A is an essential component of MTOC in mouse oocytes.
International Conference of the Korean Society for Molecular and Cellular Biology.
KOREA, REPUBLIC OF
(2015)
Inhibition of catalase affects negative effects on the proper chromosome segregation.
International Conference of the Korean Society for Molecular and Cellular Biology.
KOREA, REPUBLIC OF
(2015)
Zw10 is required for accurate chromosome segregation during meiotic maturation in mouse oocytes.
International Conference of the Korean Society for Molecular and Cellular Biology.
KOREA, REPUBLIC OF
(2015)
Inhibition of class III PI3K shows failure of cytokinesis midbody abscission but not autophagy during oocyte meiotic maturation.
한국발생생물학회.
KOREA, REPUBLIC OF
(2015)
Zwint-1 is required for homologous chromosome segregation by regulating kinetochore-microtubule attachment and spindle assembly checkpoint during meiosis of mouse oocytes.
한국발생생물학회.
KOREA, REPUBLIC OF
